Laboratory Technician I - Biochemistry Department USU - Bethesda, MD

Updated: April 10, 2020
Job ID: 215641
Status: Full-Time
Regular/Temporary: Regular
Location: Bethesda, MD, United States

Join the HJF Team!

HJF is seeking a Laboratory Technician I to support the Satpute-Krishnan lab in the Biochemistry Department located at the Uniformed Services University (USU) in Bethesda, Maryland. HJF provides scientific, technical and programmatic support services to Biochemistry Department. 

The Satpute-Krishnan lab is new cell and molecular biology lab focused on dissecting protein quality control machinery within the secretory pathway of cells and elucidating the molecular basis of protein misfolding diseases. 

Responsibilities:

  • Provide general lab support as needed 50-75% of the time as directed by the PI. This includes helping the PI obtain preliminary data for grant proposals and performing pilot studies to help initiate projects for trainees/students. Specific duties include:
    1. (For the below duties 1-5) maintain a detailed and complete electronic lab notebook.
    2. Optimize protocols for “click chemistry” experiments.
    3. Construct lab plasmids to support lab projects.
    4. Perform co-immunoprecipitations and western blots to generate preliminary results.
    5. Produce stable cell lines for general lab use.
    6. Follow and reinforce frameworks that are in place for the maintenance of lab databases, DNA construct glycerol stocks log, sequencing log and primer logs.
    7. Oversee the repair of equipment or ordering supplies as necessary.
    8. Perform communal lab-duties including maintaining cleanliness and organization of laboratory areas, aliquoting reagents, and creating new reagents for lab stocks.
  • Pursue independent research project 25-50% of the time as directed by the PI. Research project is focused on dissecting protein quality control factors in the endoplasmic reticulum (ER) that clear potentially toxic, disease-associated misfolded proteins. Specific duties include:
    1. Maintain a detailed and complete electronic lab notebook for research project.
    2. Read primary scientific literature relating to ER protein quality control and develop expertise in this field, and assemble this information into detailed referenced drafts.
    3. Write passages describing results and methods, and prepare figures for publications.
    4. Perform co-immunoprecipitations, western blots, microscopy and other techniques as required by the PI.
    5. Use computer software for data analysis.

Required Knowledge, Skills, and Abilities: Demonstrated academic mastery in lab and course work in cell biology, molecular biology and biochemistry. Strong organizational skills, strong written and verbal English communication skills. Ability to focus stringent attention on details, to work with others in a team-oriented working environment, and maintain high ethical standards. Ability to identify issues and problems, troubleshoot them independently and report the effort to PI.

Minimum Education/Training Requirements: Associate degree in biology or any of the sub-specialties (e.g. molecular biology, biochemistry, biophysics).

Minimum Experience:  0 to 2 years of experience.

Physical Capabilities: Requires long periods of standing or sitting and performing focused bench work involving tube labeling and pipetting. 

Work Environment: Laboratory environment; may require working evenings and weekends; may involve working with biohazardous materials.

Background: Eligibility to obtain a Common Access Card (CAC); eligibility to obtain and hold a Public Trust background.

Employment with HJF is contingent upon successful completion of a background check, which may include, but is not limited to, contacting your professional references, verification of previous employment, addresses, education, and credentials, a criminal background check, drug screening, and a department of motor vehicle (DMV) check.  

HJF is an equal opportunity and affirmative action employer.  All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status.

Any qualifications to be considered as equivalents, in lieu of stated minimums, require the prior approval of the Chief Human Resources Officer.
 

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