Lab Technician Supervisor

Updated: Today
Job ID: 215166
Status: Full-Time
Regular/Temporary: Regular
Location: Silver Spring, MD, United States

Join the HJF Team!

HJF is seeking a Research Assistant III/ Laboratory Technician to support the Military HIV Research Program (MHRP) located at the Walter Reed Army Institue of Research (WRAIR) in Silver Spring, Maryland. HJF provides scientific, technical and programmatic support services to MHRP. 

Responsible for day-to-day management of the laboratory. Assigns and assures that research assistants and students conduct laboratory responsibilities. Maintains inventories and orders supplies. Maintains and manages equipment. Responsible for research and/or development in collaboration with others for projects relating to HIV-1 vaccine basic research and adjuvant research. Provides laboratory and technical support to senior technical and professional staff. Works in support of projects on the development of antibodies to HIV-1, immunological assays and the mechanism of adjuvants. 

Responsbilities: 

  1. Works in immunology/biochemistry laboratory conducting experiments related to HIV vaccines and liposome-based adjuvants and other adjuvants. 
  2. Expected to work with minimal supervision on projects to be developed with the supervisory personnel.
  3. Servers as laboratory manger and instructs other technicians and students in standard experimental protocols and proper use of equipment.
  4. Works in a safe manner following all facility safety policies and ensures other laboratory staff work in a safe manner and complete general laboratory tasks.
  5. Maintains or oversee staff assigned to maintain inventories of supplies, reagents, chemical and biologicals. Oversee the ordering of these agents by laboratory staff or self. 
  6. Interfaces on behalf of laboratory personnel with WRAIR support services including medical maintenance, Datatron, medical waste disposal, equipment disposal, and lab coats.
  7. Performs laboratory research projects, including collection and organization of data. Makes detailed observations about experiments.  
  8. Develops new experimental protocols.
  9. Maintains cells in culture and freezes cell cultures including PBMC.
  10. Conducts ELISA on animal and human serum, plasma, cell culture supernatants, and ascites fluid for antibodies to lipids and HIV-1 derived recombinant proteins.
  11. Develops methods for the purification of antibodies and purifies monoclonal antibodies.
  12. Prepares liposomes formulations for immunization of animals.
  13. Runs gels and performs Western blot analysis.
  14. Isolates PBMC from leukopaks and propagates HIV-1 in PBMC and cell lines.
  15. Conducts erythrocyte lysis assays.
  16. Assists in the preparation of technical reports, summaries, and protocols.
  17. Maintains clear and accurate laboratory records, inventories and logbooks.
  18. Performs data entry for research projects and maintains databases.
  19. Graphs data for presentation.
  20. Supervises summer student staff and research assistant I and II staff.
  21. Performs other duties as assigned.

Required Knowledge, Skills, and Abilities: Knowledge of standard laboratory techniques; ability to work independently with only overall project consultation with supervisor; good communication, analytical and computer skills including word processing and spreadsheets.

Minimum Education/Training Requirements: Bachelor’s degree in a scientific discipline.

Minimum Experience: 4-6 years laboratory experience after BS degree in biochemistry or immunology laboratory; Experience with ELISA, and cell culture is helpful, but not essential.

Physical Capabilities:  Long periods of standing and sitting; handling various chemicals.

Supervisory Responsibilities/Controls: Summer student work and approval of students’ time.

Work Environment:  Laboratory environment.

Background:  Must have lived in the US 3 out of the past 5 years; eligible to pass a NACI background.

Employment with HJF is contingent upon successful completion of a background check, which may include, but is not limited to, contacting your professional references, verification of previous employment, addresses, education, and credentials, a criminal background check, drug screening, and a department of motor vehicle (DMV) check.  

HJF is an equal opportunity and affirmative action employer.  All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status.

Any qualifications to be considered as equivalents, in lieu of stated minimums, require the prior approval of the Chief Human Resources Officer.
 

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