Postdoctoral Fellow

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Updated: April 26, 2019
Job ID: 214534
Status: Full-Time
Regular/Temporary: Regular
Location: Bethesda, MD, United States

Join the HJF Team!

HJF is seeking a Postdoctoral Fellow - Informatics/Biomarker/Mouse Genetics/Cancer Immunology to support the lab of Dr. Geeta Upadhyay within the Department of Pathology located at Uniformed Services University of Health Sciences (USUHS) in Bethesda, MD. HJF provides scientific, technical and programmatic support services to USUHS. 

The postdoctoral candidate will have the opportunity to conduct some of the research described in our newly funded R01 grant title “Role of Ly6K in TGF-beta and immune escape pathways of triple negative breast cancer”. Ly6K, a GPI anchored protein, is expressed in cancer tissue and is required for in vivo tumor progression. We have shown that Ly6K activates TGF-beta signaling, epithelial-to-mesenchymal transition and PD-L1 expression. We found that knockdown or pharmacological inhibition of Ly6K by small molecules binders, can significantly inhibit multiple distinct aspects of the biological activity of Ly6K. This proposal will be focused on determining the molecular basis of Ly6K action and Ly6K targeted therapeutics. In addition, they will be provided to develop new projects focused on drug resistance and drug development focused on Ly6E and other members of this family. 

Responsibilities:

  1. Assists in designing, developing, executing, and implementing scientific research and/or development.
  2. Investigates the feasibility of applying a wide variety of scientific principles and theories to potential inventions and products.
  3. Performs specialized laboratory research utilizing experimental protocols which will involve specialized procedures such as DNA cloning and sequencing, Southern blotting, molecular analysis of specific RNA transcripts by quantitative RT-PCR or Northern analysis, animal injections, bleedings and immunizations.
  4. Collects and handles samples and keeps detailed records of experiments.
  5. Assists with the preparation of data for presentations at scientific meetings and for publication in journals.
  6. Assists with training laboratory staff as needed.
  7. Maintains cleanliness of laboratory areas.
  8. Performs other duties as assigned.

Required Knowledge, Skills, and Abilities:

  • Knowledge of appropriate scientific area; ability to analyze and interpret data.
  • Ability to independently analyze next gen sequencing data.
  • Flow cytometry: Sample prep, execution on the machine, data analysis, sorting and multicolor panel.
  • Cell culture: Primary and cell lines, T cell expansion.
  • In vivo studies: Mouse handling, i.p. injections, tumor injections and measurements, immunotherapy assays, tissue collection and processing, mouse genetics.
  • In vitro and ex vivo assays: cell signaling, live cell imaging, westerns, qRT-PCR, luminex.

Minimum Education/Training Requirements: PhD in a related scientific discipline.

Minimum Experience: 0-2 years of relevant experience. 

Physical Capabilities: Long periods of standing and sitting; intricate work with hands; carrying of light and moderately heavy laboratory equipment.

Supervisory Responsibilities/Controls: May provide guidance to laboratory staff.

Work Environment: Laboratory environment; may involve working with biohazardous materials; may require working evenings and weekends.

Background: Eligibility to obtain a Common Access Card (CAC); eligibility to obtain and hold a Public Trust background.

Employment with HJF is contingent upon successful completion of a background check, which may include, but is not limited to, contacting your professional references, verification of previous employment, addresses, education, and credentials, a criminal background check, drug screening, and a department of motor vehicle (DMV) check.  

HJF is an equal opportunity and affirmative action employer.  All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status.

Any qualifications to be considered as equivalents, in lieu of stated minimums, require the prior approval of the Chief Human Resources Officer.
 

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