Postdoctoral Fellow

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Updated: July 12, 2019
Job ID: 214379
Status: Full-Time
Regular/Temporary: Regular
Location: Bethesda, MD, United States

Join the HJF Team!

HJF is seeking Postdoctoral Fellow to explore novel therapeutics of metastatic breast cancer to support the lab of Dr. Geeta Upadhyay within the Department of Pathology located at the Uniformed Services University (USU) in Bethesda, MD. HJF provides scientific, technical and programmatic support services to the Department of Pathology.

About USUHS: The mission of the USUHS is to educate, train, and comprehensively prepare uniformed services health professionals, scientists, and leaders to support the Military and Public Health Systems, the National Security and National Defense Strategies of the United States, and the readiness of our Uniformed Services. USUHS is integral part of F. Edward Hbert School of Medicine. Within campus, we collaborated with Armed Forces Radiobiology Research Institute and with Murtha Cancer Center, Walter Reed, capital Breast care project. USUHS is located in close proximity to National Institute of health, providing opportunities for cross campus collaboration. More info: https://www.usuhs.edu/

Core facilities at USUHS: https://www.usuhs.edu/research/core-facilities

About Geeta Upadhyay: Dr. Upadhyay recently joined USUHS faculty as tenure track faculty. Dr. Upadhyay received her training at Harvard, NCI, UTHSCSA, Georgetown University. Her research is focused on biomarker discover, novel drug development and drug resistance in breast cancer. Click here for a complete list of publications: https://www.ncbi.nlm.nih.gov/myncbi/browse/collection/42266779/?sort=date&direction=descending

Project Description: The postdoctoral candidate will have the opportunity to conduct cutting edge research on novel therapeutics including opportunity to explore immunotherapy for metastatic breast cancer.  Our lab is focused on understanding the novel role of human Ly6 genes in drug resistance, cancer progression and metastasis. Please see following publication for more detailed account of the research projects.

Upadhyay G, Yin Y, Yuan H, Li X, Derynck R, Glazer RI. Stem cell antigen-1 enhances tumorigenicity by disruption of growth differentiation factor-10 (GDF10)-dependent TGF-beta signaling. Proc. Natl. Acad. Sci. U.S.A., 108, 7820–7825 (2011). PMID: 21518866.

AlHossiny M, Luo L, Frazier WR, Steiner N, Gusev Y, Kallakury B, Glasgow E, Creswell K, Madhavan S, Kumar R, Upadhyay G. Lymphocyte antigens 6 complex K/E-transforming growth factor-beta axis promotes breast cancer tumorigenesis. Cancer Res., 76, 3376–3386 (2016). PMID: 27197181.

Luo L, McGarvey P, Madhavan S, Kumar R, Gusev Y, Upadhyay G. Lymphocyte antigens 6 complex gene family members drive tumorigenesis. Oncotarget,  7, 11165–11193 (2016). PMID: 2686284

Responsibilities: 

  1. Assists in designing, developing, executing, and implementing scientific research and/or development.
  2. Investigates the feasibility of applying a wide variety of scientific principles and theories to potential inventions and products.
  3. Performs specialized laboratory research utilizing experimental protocols which will involve specialized procedures such as DNA cloning and sequencing, Southern blotting, molecular analysis of specific RNA transcripts by quantitative RT-PCR or Northern analysis, animal injections, bleedings and immunizations.
  4. Collects and handles samples and keeps detailed records of experiments.
  5. Assists with the preparation of data for presentations at scientific meetings and for publication in journals. 
  6.  Assists with training laboratory staff as needed.
  7. Maintains cleanliness of laboratory areas.
  8. Performs other duties as assigned.

Required Knowledge, Skills, and Abilities:  Knowledge of appropriate scientific area; ability to analyze and interpret data.

  • Bioinformatic analysis of large dataset
  • Flow cytometry: Sample prep, execution on the machine, data analysis, sorting and multicolor panel
  • Cell culture: Primary and cell lines, T cell expansion.
  • In vivo studies: Mouse handling, i.p. injections, tumor injections and measurements, immunotherapy assays, tissue collection and processing, mouse genetics, knockout mice model
  • In vitro and ex vivo assays: cell signaling, live cell imaging, westerns, qRT-PCR, luminex.
  • Protein purification, Elisa
  • Immunohistochemistry

Minimum Education/Training Requirements:  PhD in a related scientific discipline

Minimum Experience:  0 to 4 years directly related research experience

Physical Capabilities:  Requires long periods of sitting and standing; handling of various chemicals, ability to work weekend/evenings

Supervisory Responsibilities/Controls:  Provides guidance to lower level laboratory staff.

Work Environment:  Laboratory environment; may involve working with biohazardous materials; may require working evenings and weekends

Background: Eligibility to obtain a Common Access Card (CAC); eligibility to obtain and hold a Public Trust background.

HJF is an equal opportunity and affirmative action employer.  All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status.

Any qualifications to be considered as equivalents, in lieu of stated minimums, require the prior approval of the Chief Human Resources Officer.
 

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