Postdoctoral Research Fellow - Immuno/Oncology of Breast Cancer

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Updated: October 27, 2018
Job ID: 214129
Status: Full-Time
Regular/Temporary: Regular
Location: Bethesda, MD, United States

Join the HJF Team!

HJF is seeking a Postdoctoral Fellow in Cancer Immunology to support the lab of Dr. Geeta Upadhyay at the Department of Pathology, Uniformed Services University of Health Sciences (USUHS), Bethesda, MD. HJF provides scientific, technical and programmatic support services to USUHS.

About USUHS: The mission of the USUHS is to educate, train, and comprehensively prepare uniformed services health professionals, scientists, and leaders to support the Military and Public Health Systems, the National Security and National Defense Strategies of the United States, and the readiness of our Uniformed Services. USUHS is integral part of F. Edward Hébert School of Medicine. Within campus, we collaborated with Armed Forces Radiobiology Research Institute and with Murtha Cancer Center, Walter Reed, capital Breast care project. USUHS is located in close proximity to National Institute of health, providing opportunities for cross campus collaboration. More info: https://www.usuhs.edu/

Core facilities at USUHS: https://www.usuhs.edu/research/core-facilities

About Geeta Upadhyay: Dr. Upadhyay recently joined USUHS faculty as tenure track faculty. Dr. Upadhyay received her training at Harvard, NCI, UTHSCSA, Georgetown University. Her research is focused on biomarker discover, drug development and drug resistance in breast cancer.

Click here for a complete list of publications.

PROJECT DESCRIPTION: The postdoctoral candidate will have the opportunity to conduct some of the research described in our newly funded R01 grant title “Role of Ly6K in TGF-beta and immune escape pathways of triple negative breast cancer”. Ly6K, a GPI anchored protein, is expressed in cancer tissue and is required for in vivo tumor progression. We have shown that Ly6K activates TGF-beta signaling, epithelial-to-mesenchymal transition and PD-L1 expression. We found that knockdown or pharmacological inhibition of Ly6K by small molecules binders, can significantly inhibit multiple distinct aspects of the biological activity of Ly6K. This proposal will be focused on determining the molecular basis of Ly6K action and Ly6K targeted therapeutics.   

RESPONSIBILITIES                                                                                                90% of time

1.  Assists in designing, developing, executing, and implementing scientific research and/or development.

2.  Investigates the feasibility of applying a wide variety of scientific principles and theories to potential inventions and products.

3.  Performs specialized laboratory research utilizing experimental protocols which will involve specialized procedures such as DNA cloning and sequencing, Southern blotting, molecular analysis of specific RNA transcripts by quantitative RT-PCR or Northern analysis, animal injections, bleedings and immunizations.

4.  Collects and handles samples and keeps detailed records of experiments.

5.  Assists with the preparation of data for presentations at scientific meetings and for publication in journals. 

NONESSENTIAL JOB DUTIES:                                                                          10% of time

6.  Assists with training laboratory staff as needed.

 7.  MaintaiNs cleanliness of laboratory areas.

8.  Performs other duties as assigned.

JOB SPECIFICATIONS:

Required Knowledge, Skills, and Abilities:  Knowledge of appropriate scientific area; ability to analyze and interpret data.

  • Flow cytometry: Sample prep, execution on the machine, data analysis, sorting and multicolor panel
  • Cell culture: Primary and cell lines, T cell expansion.
  • In vivo studies: Mouse handling, i.p. injections, tumor injections and
  • measurements, immunotherapy assays, tissue collection and processing, mouse genetics.
  • In vitro and ex vivo assays: cell signaling, live cell imaging, westerns, qRT-PCR, luminex.

Minimum Education/Training Requirements:  PhD in a related scientific discipline

Minimum Experience:  0-2 years

PhYsical Capabilities:  Long periods of standing and sitting; intricate work with hands; carrying of light and moderately heavy laboratory equipment

Supervisory Responsibilities/Controls:  May provide guidance to laboratory staff

Work Environment:  Laboratory environment; may involve working with biohazardous materials; may require working evenings and weekends

HJF is an equal opportunity and affirmative action employer.  All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status.

Any qualifications to be considered as equivalents, in lieu of stated minimums, require the prior approval of the Director of Human Resources

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