Research Assistant III (Enteric Diseases Department)

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Updated: January 27, 2018
Job ID: 213431
Status: Full-Time
Regular/Temporary: Regular
Location: Silver Spring, MD, United States

Come Work at HJF!

HJF is seeking a Research Assistant III (RAIIII) to support the Military Enteric Diseases Research Program, located at the Naval Medical Research Center (NMRC) in Silver Spring, Maryland.  HJF provides scientific, technical and programmatic support services to NMRC.

Enterotoxigenic E. coli (ETEC) causes diarrhea and is among the Military priorities for vaccine development. The ETEC Vaccine Program works on several facets of vaccine development, from epidemiological studies, to antigen design and evaluation, to phase 1 and 2 clinical trials. The Immunology Group is responsible for all preclinical (mouse studies) as well clinical (clinical trials) vaccine development activities. The incumbent will join a team of two scientists and four RAs in charge of activities in the immunology laboratory.

The successful candidate should have at least four years of experience of hands-on experience in immunologic assays. Beneficial experience includes cell culture to evaluate T cell proliferation and cytokine secretion, ELISPOT for assessment of T and B cell responses, and multiparametric flow cytometry for T and B cell profiling.  


  1. Overall laboratory organization, preparation of tubes, racks, labels, media and solutions and supplies.
  2. Log in, label, separate, and distribute human samples for processing.
  3. Process serum, saliva, and cell samples (Ficoll-based PBMC isolation), and cell supernatants.
  4. Perform enzyme-based serum and cell assays (e.g. ELISA, ELISPOT)).
  5. Perform, scan, quality-control, and analyze ELISPOT assays.
  6. Set up and complete cell culture for different goals, such as supernatant collection, flow cytometry evaluations, or memory B cell assays.
  7. Count, distribute, stain and acquire cells in flow cytometry equipment (e.g. Fortessa, BD).
  8. Perform data analysis, prepare graphical representation of data (GraphPad Prism™ software).
  9. Organize sample databases and freezer inventories (Windows Word® and Excel®).
  10. Complete other projects and duties as assigned.

Required Knowledge, Skills, and Abilities:  4-6 years of of hands-on experience in immunologic assays. Knowledge of highly complex scientific procedures, techniques and fundaments; knowledge and practical experience in serum and PBMC isolation. Beneficial experience: Cellular immunological assays (ELISPOT, cell culture, flow cytometry, cytokine measurement). Practical experience in human clinical trials, specifically in the immunology area. Should be able to follow directions, interpret and follow SOPs and SSPs, work in a team as well as independently.

Minimum Education/Training Requirements:  BS or BA required in a biology-related field (e.g. immunology, microbiology, biochemistry). Preferred: Master’s degree in immunology.

 Physical Capabilities:  Work involves long periods of standing, handling numerous chemicals and biological samples.   

Work Environment:  Laboratory environment; requires working with human specimens including cells, plasma/serum, saliva, and stool; may require working long hours.

 Background: This position will require fingerprinting for eligibility to work at NMRC. Must be eligible to obtain Public Trust clearance.

HJF is an equal opportunity and affirmative action employer.  All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status. Any qualifications to be considered as equivalents, in lieu of stated minimums, require the prior approval of the Chief Human Resources Officer.